Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Dipeptidyl peptidase 3 (also known as DPP3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat DPP3 antibody. DPP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat DPP3 Antibody is added and binds to DPP3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DPP3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat DPP3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm